{"id":293,"date":"2014-05-13T16:48:51","date_gmt":"2014-05-13T13:48:51","guid":{"rendered":"http:\/\/garinilab.biu.ac.il\/?page_id=293"},"modified":"2014-05-13T16:48:51","modified_gmt":"2014-05-13T13:48:51","slug":"protein-conformations-studied-at-single-molecule-level","status":"publish","type":"page","link":"https:\/\/garinilab.biu.ac.il\/?page_id=293","title":{"rendered":"Protein conformations studied at single molecule level"},"content":{"rendered":"<p>\t\t\t\tProteins can be viewed as nano-machines with moving elements. We are studying the link between conformational changes that a folded protein undergoes and its functionality at high temporal resolution.<br \/>\nThe model molecule presently used for this study is the <i>E. Coli<\/i> Adenylate kinase; a multi-domain enzyme molecule.<br \/>\nThe research is performed at the single molecule level in high temporal resolution achieved by applying different fluorescence techniques such as <a href=\"http:\/\/en.wikipedia.org\/wiki\/F%C3%B6rster_resonance_energy_transfer\" target=\"_blank\" rel=\"noopener noreferrer\">FRET <\/a>and <a href=\"http:\/\/en.wikipedia.org\/wiki\/Fluorescence_correlation_spectroscopy\" target=\"_blank\" rel=\"noopener noreferrer\">FCS<\/a>. The project is done as a collaboration with <a class=\"one\" href=\"http:\/\/www.biu.ac.il\/LS\/People\/staff\/Haas\/\"> Haas&#8217; group<\/a>.<\/p>\n<div id=\"attachment_161\" style=\"width: 231px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/05\/AK1.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-161\" class=\"wp-image-161 size-full\" src=\"https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/05\/AK1.jpg\" alt=\"Adenylate kinase\" width=\"221\" height=\"222\" srcset=\"https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/05\/AK1.jpg 221w, https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/05\/AK1-150x150.jpg 150w\" sizes=\"(max-width: 221px) 100vw, 221px\" \/><\/a><p id=\"caption-attachment-161\" class=\"wp-caption-text\">Adenylate kinase<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Proteins can be viewed as nano-machines with moving elements. We are studying the link between conformational changes that a folded protein undergoes and its functionality at high temporal resolution. The model molecule presently used for this study is the E. Coli Adenylate kinase; a multi-domain enzyme molecule. The research is performed at the single molecule [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":94,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=\/wp\/v2\/pages\/293"}],"collection":[{"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=293"}],"version-history":[{"count":0,"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=\/wp\/v2\/pages\/293\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=\/wp\/v2\/pages\/94"}],"wp:attachment":[{"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=293"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}