{"id":808,"date":"2014-04-24T07:52:44","date_gmt":"2014-04-24T07:52:44","guid":{"rendered":"http:\/\/garinilab.biu.ac.il\/?page_id=2"},"modified":"2014-04-24T07:52:44","modified_gmt":"2014-04-24T07:52:44","slug":"sample-page-2","status":"publish","type":"page","link":"https:\/\/garinilab.biu.ac.il\/","title":{"rendered":"Home"},"content":{"rendered":"<div id=\"overall_counter\">\n<h3>Studying the Physics of Biological Systems &#8211; Bio &#8211; Nano &#8211; Photonics<\/h3>\n<h3>What is biophysics?<\/h3>\n<p>Schr\u00f6dinger touched a fascinating question about the physical nature of life in his book &#8216;<em>What is Life? The Physical Aspect of the Living Cell<\/em>&#8216;:<img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-8\" src=\"https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/04\/What-is-life-Schrodinger.jpg\" alt=\"What is life - Schrodinger\" width=\"203\" height=\"164\" \/><\/p>\n<blockquote><p><i>&#8220;The question is: How can the events in space and time which take place within the spatial boundary of a living organism be accounted for by physics and chemistry?&#8221;<\/i><\/p><\/blockquote>\n<p>The attempt to answer this question still continues to date with great success.\u00a0Biophysics\u00a0is an interdisciplinary science that tries to explain biological phenomena with physical models and to develop novel physical tools for biological studies. By studying biological systems with this multidisciplinary approach, new insights can be achieved. In addition to the exciting work, new discoveries will surely lead to improved human welfare.<\/p>\n<h3>Research topics:<\/h3>\n<p>[tabs]<br \/>\n[tab title=&#8221;Single-molecules&#8221;]<\/p>\n<table>\n<tbody>\n<tr>\n<td style=\"width: 255px;\"><a title=\"3D Tethered Particle Motion (TPM) for DNA-Protein Interactions Study\" href=\"http:\/\/labs.biu.ac.il\/garinilab\/research-2\/3d-tethered-particle-motion-tpm-for-dna-protein-interactions-study\/\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-791\" src=\"https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/06\/Picture-006.jpg\" alt=\"\" width=\"196\" height=\"262\" srcset=\"https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/06\/Picture-006.jpg 2736w, https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/06\/Picture-006-225x300.jpg 225w, https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/06\/Picture-006-768x1024.jpg 768w, https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/06\/Picture-006-1568x2091.jpg 1568w\" sizes=\"(max-width: 196px) 100vw, 196px\" \/><\/a><\/td>\n<td><strong>Tethered particle motion (TPM) <\/strong>is one of our single-molecule experimental techniques, for <em>in-vitro<\/em> studies. Some of our TPM projects:<\/p>\n<ul>\n<li><a title=\"Protein-DNA Interactions\" href=\"http:\/\/labs.biu.ac.il\/garinilab\/research-2\/protein-dna-interaction\/\">DNA-HU protein interactions<\/a><\/li>\n<li><a title=\"Single molecule studies of nucleosomes dynamics in vitro\" href=\"http:\/\/labs.biu.ac.il\/garinilab\/research-2\/single-molecule-studies-of-nucleosomes-dynamics-in-vitro\/\">Nucleosomes studies<\/a><\/li>\n<li><a title=\"Diffusing particles in a harmonic potential field\" href=\"http:\/\/labs.biu.ac.il\/garinilab\/research-2\/diffusing-particles-in-a-harmonic-potential-field\/\">Diffusing particles in a potential field<\/a><\/li>\n<li><a title=\"Force-free Single molecule unfolding and refolding\" href=\"http:\/\/labs.biu.ac.il\/garinilab\/research-2\/force-free-single-molecule-unfolding-and-refolding\/\">Protein unfolding\/refolding<\/a><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/tab]<br \/>\n[tab title=&#8221;<em>in-vivo<\/em> studies&#8221;]<\/p>\n<table style=\"height: 278px;\" width=\"624\">\n<tbody>\n<tr>\n<td style=\"width: 315px;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-804\" src=\"https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/06\/IMG_20140615_141856.jpg\" alt=\"Confocal microscope\" width=\"238\" height=\"178\" srcset=\"https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/06\/IMG_20140615_141856.jpg 3264w, https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/06\/IMG_20140615_141856-300x225.jpg 300w, https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/06\/IMG_20140615_141856-768x576.jpg 768w, https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/06\/IMG_20140615_141856-1024x768.jpg 1024w, https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/06\/IMG_20140615_141856-1568x1176.jpg 1568w\" sizes=\"(max-width: 238px) 100vw, 238px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>The confocal microscope<\/strong> gives better resolution than standard microscope, but the real advantage\u00a0 is the ability to capture 3-dimensional images of living cells.<\/td>\n<td>Among the projects that use the confocal microscope:<\/p>\n<ul>\n<li><a title=\"Organization of the genome in the nucleus\" href=\"http:\/\/labs.biu.ac.il\/garinilab\/research-2\/organization-of-the-genome-in-the-nucleus\/\">Organization of telomers in the nucleus.<\/a><\/li>\n<li><a title=\"Investigating transcription at the single-copy gene level\" href=\"http:\/\/labs.biu.ac.il\/garinilab\/research-2\/investigating-transcription-at-the-single-copy-gene-level\/\">Studying single-copy gene transcription.<\/a><\/li>\n<li><a title=\"Studying the nuclear environment through chromatin diffusion\" href=\"http:\/\/labs.biu.ac.il\/garinilab\/research-2\/studying-the-nuclear-environment-through-chromatin-diffusion\/\">Anomalous diffusion in the nucleus.<i style=\"margin: 0px; padding: 0px; border: 0px; font-size: 14px; vertical-align: baseline; color: #444444; font-family: 'Open Sans', Helvetica, Arial, sans-serif; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: 24px; orphans: auto; text-align: justify; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff;\"><span class=\"Apple-converted-space\"><br \/>\n<\/span><\/i><\/a><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/tab]<br \/>\n[tab title=&#8221;Protein dynamics&#8221;]<\/p>\n<table>\n<tbody>\n<tr>\n<td style=\"width: 290px;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-807\" src=\"https:\/\/garinilab.biu.ac.il\/wp-content\/uploads\/2014\/06\/IMG_20140615_142104.jpg\" alt=\"PicoQuant \" width=\"212\" height=\"281\" \/><\/td>\n<td>The <strong>PicoQuant<\/strong> setup has the capability of measuring the fluorescence life time of single molecules. This can be used for experimental methods such as <strong>FRET<\/strong>, <strong>FLIM<\/strong> and <strong>FCS<\/strong>. The PicoQuant setup is serving us for:<\/p>\n<ul>\n<li><a title=\"Studying the mechanism of splicing factors using in-vivo dynamic methods\" href=\"http:\/\/labs.biu.ac.il\/garinilab\/research-2\/studying-the-mechanism-of-splicing-factors-using-in-vivo-dynamic-methods\/>Studying splicing factors.<\/a><\/li>\n<li><a title=\"Protein conformations studied at single molecule level\" href=\"http:\/\/labs.biu.ac.il\/garinilab\/research-2\/protein-conformations-studied-at-single-molecule-level\/\">Observation of protein conformational changes.<\/a><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/tab]<br \/>\n[\/tabs]<\/p>\n<h3><iframe loading=\"lazy\" src=\"http:\/\/www.youtube.com\/embed\/pC-dCI3wys8\" width=\"468\" height=\"250\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/h3>\n<p style=\"padding-left: 30px;\"><span style=\"font-size: 10pt;\"><em><b>Yuval Garini&#8217;s lab.<\/b><\/em><\/span><\/p>\n<p style=\"padding-left: 30px;\"><span style=\"font-size: 10pt;\"><em> <b>This video is part of the <a href=\"https:\/\/www.youtube.com\/watch?v=l9psXd_fNoU\" target=\"_blank\" rel=\"noopener noreferrer\">APS video about the physics department at Bar-Ilan university<\/a>.<\/b><\/em><\/span><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Studying the Physics of Biological Systems &#8211; Bio &#8211; Nano &#8211; Photonics What is biophysics? Schr\u00f6dinger touched a fascinating question about the physical nature of life in his book &#8216;What is Life? The Physical Aspect of the Living Cell&#8216;: &#8220;The question is: How can the events in space and time which take place within the [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=\/wp\/v2\/pages\/808"}],"collection":[{"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=808"}],"version-history":[{"count":1,"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=\/wp\/v2\/pages\/808\/revisions"}],"predecessor-version":[{"id":1092,"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=\/wp\/v2\/pages\/808\/revisions\/1092"}],"wp:attachment":[{"href":"https:\/\/garinilab.biu.ac.il\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=808"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}